PIF3 is a transcription factor that negatively regulates light responses and chlorophyll biosynthesis in plants. The study found that pif3 mutant seedlings grown in darkness expressed higher levels of genes involved in chlorophyll biosynthesis and photosynthesis compared to wild-type seedlings. They also accumulated more protochlorophyllide, a precursor to chlorophyll. When transferred to light, the pif3 mutant seedlings underwent more severe photobleaching than wild-type due to overaccumulation of protochlorophyllide in the dark. A quadruple mutant lacking four phytochrome-interacting transcription factors, including PIF3, displayed constitutive photomorphogenesis even in darkness. This suggests that phytochromes promote photomorphogenesis

Phytochromes Promote Seedling Light Responses by Inhibiting Four Negatively-Acting Phytochrome-Interacting Factors PDF

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