Protein  Characterization/ Purification Dr.  Kevin  Ahern Protein  Purification • Applications of Biochemistry Knowledge Protein  Purification • Applications of Biochemistry Knowledge • Opening Cells Protein  Purification • Applications of Biochemistry Knowledge • Opening Cells • Centrifugation Protein  Purification • Applications of Biochemistry Knowledge • Opening Cells • Centrifugation • Fractionation Dialysis • Applications of Biochemistry Knowledge Dialysis • Applications of Biochemistry Knowledge • Separates salts from proteins Chromatography  (Column) • Applications of Biochemistry Knowledge Chromatography  (Column) • Applications of Biochemistry Knowledge • Separation based on charge - Ion Exchange Chromatography  (Column) • Applications of Biochemistry Knowledge • Separation based on charge - Ion Exchange • Separation based on size - Size Exclusion / Gel Filtration